版纳微型猪近交系DLDH基因编码区序列扩增及生物信息学分析

doi:10.11843/j.issn.0366-6964.2015.10.002

Abstract

Abstract:

The study aimed to amplify the coding region of DLDH gene，which could be used to further study the signal pathway of DLDH expression and the correlation between DLDH gene expression and sperm capacitation，acrosome reaction.RNA was extracted from testicle and cDNA was obtained by reverse transcription.Specific primers were designed to amplify the coding region of DLDH gene.The sequence of coding region and amino acid of DLDH were analyzed and predicted by Lasergene，BioEdit，ClustalX，Mega bio-softs，and the molecular phylogenetic trees of coding amino acids region of DLDH gene was constructed by NJ and ME，respectively.The sequence length of coding region of the BMI DLDH gene was 1 530 bp，encoding a protein of 509 amino acids.By biology software analysis，the nucleotide and amino acid sequences of DLDH among different species were conserved in evolution.The DLDH protein contained the abundant phosphorylated sites and was assembled with α-helixes and random coil.

CLC Number:

S814

CHENG Wen-min，PAN Wei-rong，YIN Jun-fang，HUANG Yan，ZHA Xing-qin，XIAO Jing，ZENG Yang-zhi. Amplification and Bioinformatics Analysis of Coding Region of the DLDH Gene in Banna Mini-pig Inbred Line (BMI)[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(10): 1706-1712.

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Amplification and Bioinformatics Analysis of Coding Region of the DLDH Gene in Banna Mini-pig Inbred Line (BMI)

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